As described for oncogenes the Raf and Src kinases were analyzed predominantly to understand molecular mechanism of proliferation, differentiation and metastasis formation.

For links to publications see Oncogenes and Cancer

Publications about kinases

  1. Owada, M., Donner, P., Dittmar, K.E.J. and Moelling, K. Comparison of protein kinase activities in normal cells and cells transformed by a temperature-sensitive mutant of avian sarcoma virus to those of cell-free viral translation products. Cold Spring Harbor Symp. Quant. Biol. 44, 959-965 (1980).
  2. Owada, M. and Moelling, K.: Temperature-sensitive kinase activity associated with various mutants of avian sarcoma viruses which are temperature sensitive for transformation. Virology 101, 157-168 (1980).
  3. Owada, M.K., Donner, P., Scott, A. and Moelling, K. Isolation of an avian sarcoma virus-specific protein kinase from virus particles. Virology 110, 333-343 (1981).
  4. Bunte, T., Owada, M.K., Donner, P., Boschek, C.B. and Moelling, K. Association of the transformation-specific protein pp60src with the membrane of an avian sarcoma virus. J. Virol. 38, 1034-1047 (1981).
  5. Donner, P., Bunte, T., Owada, M.K. and Moelling, K. Biochemical characterization of pp60src-associated protein kinase from avian sarcoma virus Schmidt-Ruppin strain. J. Biol. Chem. 256, 8786-8794 (1981).
  6. Moelling, K., Owada, M.K., Greiser-Wilke, I., Bunte, T. and Donner, P. Biochemical characterization of transformation-specific proteins of acute avian leukemia and sarcoma viruses. J. Cell. Biochem. 20, 63-69 (1982).
  7. Steinheider, G., Greiser-Wilke, I., Hauser, H., Bumke-Vogt, C., Moelling, K. and Graessmann, A. DNA-mediated gene transfer in Friend leukemia cells by cotransfection of Simian Virus 40 DNA with Herpes simplex virus thymidine kinase DNA. J. Virol. 45, 375-382 (1983).
  8. ***Moelling, K., Heimann, B., Beimling, P., Rapp, U.R. and Sander, T.: Serine- and threonine-specific protein kinase activities of purified gag-mil and gag-raf proteins. Nature 312, 558-561 (1984).
  9. Kurth, R., Tanaka, T. and Moelling, K. Isolation of monoclonal antibodies specific for Rous Sarcoma Virus structural, polymerase and transforming proteins and their use for the study of mutant virus-infected cells. J. Gen. Virol. 66, 827-837 (1985).
  10. Heimann, B., Beimling, P., Pfaff, E., Schaller, H. and Moelling, K. Analysis of a tyrosine-specific protein kinase activity associated with the retroviral erbB oncogene product. Exp. Cell Res. 161, 199-208 (1985).
  11. Denhez, F., Heimann, B., d’Auriol, L., Graf, T., Coquillaud, M., Coll, J., Galibert, F., Moelling, K., Stehelin, D. and Ghysdael, J. Replacement of lys 622 in the ATP binding domain of P100gag-mil abolishes the in vitro autophosphorylation of the protein and the biological properties of the v-mil oncogene of MH2 virus. EMBO J. 7, 541-546 (1988).
  12. Haeder, M., Rotsch, M., Bepler, G., Hennig, C., Havemann, K., Heimann, B. and Moelling, K. Epidermal growth factor receptor expression in human lung cancer cell lines. Cancer Research 48, 1132-1136 (1988).
  13. Denhez, F., Galibert, F., Heimann, B., Dozier, C., Graf, T., Moelling, K., Stehelin, D. and Ghysdael, J. An invariant asparagine residue belonging to a highly conserved domain in all protein kinases is instrumental in the protein kinase activity of the v-mil gene product. Oncogene Research 3, 199-205 (1988).
  14. Beimling, P. and Moelling, K. Direct interaction of CREB protein with 21 bp Tax-response elements of HTLV-1 LTR. Oncogene 7, 257-262 (1992).
  15. Lovric, J., Bischoff, O. and Moelling, K. Cell cycle-dependent association of Gag-Mil and hsp90. FEBS Letters 343, 15-21 (1994).
  16. Schramm, K., Niehof, M., Radziwill, G., Rommel, C. and Moelling, K. Phosphorylation of c-Raf-1 by protein kinase A interferes with activation. Biochem. Biophys. Res. Com. 201, 740-747 (1994).
  17. Niehof, M., Beimling, P. and Moelling, K. The aminoterminus of c-Raf-1 binds a 28 kD cellular protein. Biochem. Biophys. Res. Com. 203, 1560-1566 (1994).
  18. **Mueller, G., Strack, B., Dannull, J., Sproat, B., Surovoy, A., Jung, G. and Moelling, K. Amino Acid Requirements of the nucleocapsid Protein of HIV-1 Increases Catalytic Activity of Ki-ras Ribozyme in vitro. J. Mol. Biol. 242, 422-429 (1994).
  19. Niehof, M., Radziwill, G., Klauser, S. and Moelling, K. A small petptide derived from aminoterminus of c-Raf-1 inhibits c-Raf-1/Ras binding. Biochem. Biophys. Res. Com. 206, 46-50 (1995).
  20. Beimling, P., Niehof, M., Radziwill, G. and Moelling, K. The aminoterminus of c-Raf-1 binds a protein kinase phosphorylation Ser259. Biochem. Biophys. Res. Com. 204, 841-848 (1994).
  21. Ratziwill, G., Niehof, M., Rommel, C. and Moelling, K. Direct interaction and aminoterminal phosphorylation of c-Jun by c-Mil/Raf. Proc. Natl. Acad. Sci. USA 92, 1421-1425 (1995).
  22. Moelling, K., Strack, B. and Radziwill, G. Signal Transduction as Target of Gene Therapy. In: “New Perspectives in Molecular and Clinical Management of Gastrointestinal Tumors” (E.D. Kreuser and P.M. Schlag, eds.), Springer-Verlag Berlin Heidelberg (1996).
  23. Rommel, C., Radziwill, G., Lovric’, J., Noeldeke, J., Heinicke, T., Jones, D., Aitken, A. and Moelling, K. Activated Ras displaces 14-3-3 protein from the amino terminus of c-Raf-1. Oncogene 12, 609-619 (1996).
  24. Lovric’, J. and Moelling, K. Activation of Mil/Raf protein kinases in mitotic cells. Oncogene 12, 1109-1116 (1996).
  25. Pavlovic, J., Nawrath, M., Tu, R., Heinicke, T. and Moelling, K. Anti-tumor Immunity is involved in the thymidine kinase-mediated killing of tumors induced by activated Ki-ras (G12V). Gene Therapy 3, 635-643 (1996).
  26. Vorbrueggen, G., Lovric’, J. and Moelling, K. Functional analysis of phosphorylation at serine 532 of human c-Myb by MAP-kinase. Biol. Chem. 377, 721-730 (1996).
  27. Radziwill, G., Steinhusen, U., Aitken, A. and Moelling, K. Inhibition of Raf/MAPK signaling in Xenopus oocyte extracts by Raf-1 specific peptides. Biochem. Biophys. Res. Com. 227, 20-26(1996).
  28. Rommel, C., Radziwill, G., Moelling, K. and Hafen, E. Negative regulation of Raf activity by binding of 14-3-3 to the amino terminus of Raf in vivo. Mechanisms of Development 64, 95-104 (1997).
  29. Zimmermann S., Rommel C., Ziogas A., Lovric’ J., Moelling K. and Radziwill G. MEK1 mediates a positive feedback on Raf-1 activity independently of Ras and Src. Oncogene 15, 1503-1511 (1997).
  30. Dubois. 14-3-3 Is phosphorylated by casein kinase I on residue 233.J. Biol. Chem., 272: 28882-28888 (1997).
  31. Ziogas, A., Lorenz, I. C., Moelling K. and Radziwill G. Mitotic Raf-1 is stimulated independently of Ras and is active in the cytoplasm. J. Biol. Chem. 273, 24108-24114 (1998).
  32. Rothmann, K., Schnoelzer, M., Radziwill, G., Hildt, E., Moelling, K. and Schaller, H. Host cell-virus cross talk: phosphorylation of a Hepatitis B virus envelope protein mediates intracellular signaling. Journal of Virology 72, 10138-10147 (1998).
  33. *Buchert, M., Schneider, S., Adams, M., Baechi, T., Moelling, K. and Hovens, C.M. The junction-associated protein AF-6 interacts and clusters with specific Eph receptor tyrosine kinase at specialized sites of cell-cell-contact in the brain. J. Cell Biol 144, 361-371 (1999).
  34. ***Schneider, S., Buchert, M., Adams, M., Baechi, T., Moelling, K. and Hovens, C.M. Mutagenesis and selection of PDZ domains able to bind to new protein targets. Nature Biotechnology 17, 170-175 (1999).
  35. ***Rommel, Ch., Clarke, B.A., Zimmermann, S., Nunez, L., Rossman, R., Reid, K., Moelling, K., Yancopoulos, G.D. and Glass, D.J. Differentiation stage-specific inhibition of the Raf-MEK-ERK pathway by Akt. Science 286, 1738-1741 (1999).
  36. ***Zimmermann, S. and Moelling, K. Phosphorylation and regulation of Raf by Akt (protein kinase B). Science 286, 1741-1744 (1999).
  37. Heinicke, T., Radziwill, G., Nawrath, M., Rommel, C., Pavlovic, J. and Moelling, K. Retroviral gene transfer of dominant negative Raf-1 mutants suppresses Ha-ras-induced transformation and delays tumor formation. Cancer Gene Therapy 7, 697-706 (2000).
  38. Heinrich, J., Bosse, M., Eickhoff, H., Nietfeld, W., Reinhardt, R., Lehrach, H. and Moelling, K. Induction of putative tumor-suppressing genes in Rat-1 fibroblasts by oncogenic Raf-1 as evidenced by robot-assisted subtractive hybridization. Journal of Molecular Medicine 78, 380-388 (2000).
  39. Reusch, H.P., Zimmermann, S., Schaefer, M., Paul, M., Moelling, K. Regulation of Raf by Akt controls growth and differentiation in vascular smooth muscle cells. J. Biol. Chem. 276, 33630-33637 (2001).
  40. Moelling, K., Schad, K., Bosse, M., Zimmermann, S., Schweneker, M. Regulation of Raf-Akt cross-talk. J. Biol. Chem. 34, 31099-31106 (2002).
  41. Ladell, K., Heinrich, J., Schweneker, M., Moelling, K. A combination of plasmid DNAs encoding murine Flk-1 extracellular domain, murine IL-12 and murine IP-10 lead to tumor regression and survival in melanoma-bearing mice. J. Mol. Med. 81, 271-278 (2003).
  42. Radziwill, G., Erdmann, R.A., Margelisch, U., Moelling K. The Bcr kinase downregulates Ras signaling by phosphorylating AF-6 and binding to its PDZ domain. Molecular and Cellular Biology 23, 13, 4663-4672 (2003).
  43. Boisguerin, P., Leben. R., Ay. B., Radziwill G., Moelling K., Dong, L., Volkmer-Engert R. An Improved Method for the Synthesis of Cellulose Membrane-Bound Peptides with Free C Termini is Useful for PDZ Domain Binding Studies Chemistry & Biology 11, 449-459 (2004).
  44. Pap, Th., Nawrath, M., Heinrich, J., Bosse, M., Baier, A., Hummel, K.M., Petrow, P., Kuchen, S., Michel, B.A., Gay, R.E., Müller-Ladner, U., Moelling K., Gay St. Cooperation of Ras- and Myc-dependent pathways in regulating the growth and invasiveness of synovial fibroblasts in rheumatoid arthritis Arthritis & Rheumatism 50, 9, 2794-2802 (2004).
  45. Ziogas, A., Moelling, K. and Radziwill, G. CNK1 is a scaffold protein that regulates Src-mediated Raf-1 activation. J Biol Chem. 280, 25, 24205-24211 (2005).
  46. **Ress, A., Moelling, K. Bcr is a negative regulator of the Wnt signalling pathway. EMBO reports 6, 11, 1095-1100 (2005).
  47. Ress, A. and Moelling, K. Bcr interferes with β-catenin-Tcf1 interaction FEBS Letters 580, 1227-1230 (2006).
  48. Fritzius, Th., Burkard, G., Haas, E., Heinrich, J., Schweneker, M., Bosse, M., Zimmermann, S., Frey, A.D., Caelers, A., Bachmann, A.S. and Moelling, K. A WD-FYVE protein binds to the kinases Akt and PKCζ/λ. Biochemical Journal, 399, 9-20 (2006).
  49. Fritzius, Th., Frey, A.D., Schweneker, M., Mayer, D. and Moelling, K. WD- repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Cζ. FEBS Journal 274, 1552-1566 (2007).
  50. **Radziwill, G., Weiss, A., Heinrich, J., Baumgartner, M., Boisguerin, P., Owada, K. and Moelling, K. Regulation of c-Src by binding to the PDZ domain of AF-6. EMBO J, 26, 2633-2644 (2007).
  51. Usluoglu, N., Pavlovic, J., Moelling, K. and Radziwill, G. RIP2 mediates LPS-induced p-38 and IκBα signaling including IL-12 p40 expression in human monocyte-derived dendritic cells Eur. J. Immunol. 37, 2317-2325 (2007).
  52. Ortner, E. and Moelling, K. Heteromeric complex formation of ASK2 and ASK1 regulates stress-induced signaling. Biochem. Biophys. Res. Commun. 362, 454–459 (2007).
  53. Weiss, A., Baumgartner, M., Radziwill, G. Dennler, J. and Moelling, K. c-Src is a PDZ-interacting partner and substrate of the E3 ubiquitin ligase ligand-of-numb protein X1. FEBS Lett. 581, 5131-5136 (2007).
  54. Boisguerin, P., Ay, B., Radziwill, G., Fritz, R.D., Moelling, K. and Volkmer, R. Characterization of a Putative Phosphorilation Switch: Adaption of SPOT Synthesis to analyze PDZ Domain Regulation Mechanisms ChemBioChem 8, 2302-2307 (2007).
  55. **Baumgartner, M., Radziwill, G. Lorger, M., Weiss, A. and Moelling, K. c-Src-mediated epithelial cell migration and invasion regulated by PDZ binding site. Molecular and Cellular Biology, 28, 642-655 (2008).
  56. Fritzius, Th. and Moelling, K. Akt- and Foxo1- interacting WD-repeat-FYVE protein promotes adipogenesis EMBO J 27, 1399-1410 (2008).
  57. Ress, A. and Moelling, K. The PDZ-protein Erbin modulates β-catenin-dependent transcription Eur Surg Res. 41 (3), 284-289 (2008).
  58. **Baumgartner, M., Weiss, A., Fritzius, T., Heinrich, J., and Moelling, K. The PDZ protein MPP2 interacts with c-Src in epithelial cells. Experimental Cell Research 315: 2888-2898 (2009).
  59. Hong, S.K., Yoon S., Moelling, C. and Park J.I. Noncatalytic function of ERK1/2 can promote Raf/MEK/ERK-mediated growth arrest signaling. J Biol Chem. 184 (48): 33006-18 (2009).